SHORT ANSWER
No, not as one continuous flight. Every pet moving from the United States to Sicily lands first at Rome, because Rome Fiumicino is one of only two Italian airports authorized to carry out the official veterinary entry check for pets arriving from outside the EU (the other is Milan Malpensa). Once a pet clears that check in Rome, getting to Sicily is a second, separate leg: either a domestic ITA Airways flight from Rome to Catania, or a road transport that includes a car ferry crossing the Strait of Messina. That second leg is domestic travel within Italy, not a second import process, but it does need its own booking, its own timeline, and its own rest stop for the animal.
Sicily is part of Italy and part of the European Union, but it does not have an airport designated to perform the veterinary documentary and identity checks required for pets entering the EU from a non-EU country like the United States. Under the EU's rules for bringing a pet in from outside the bloc, every animal has to pass through a travellers' point of entry designated by the destination country, where an official checks the microchip, the rabies vaccination record, and the health certificate before the pet is legally allowed to continue its journey (European Commission, Bringing a pet into the EU from a non-EU country). In Italy, that inspection is concentrated at Rome Fiumicino and Milan Malpensa. If your final destination is Catania, Palermo, or anywhere else in Sicily, your pet's paperwork and your flight booking still need to route through one of those two mainland airports first.
This is different from a human passenger's experience, where a connecting itinerary can sometimes feel seamless. For a pet, the airport where you land from the US is the airport where the actual import happens. Everything after that is a second, domestic move.
Once the pet has passed through the travellers' point of entry in Rome and the paperwork has been reviewed, the animal is legally within Italy and the EU. The EU's own guidance on non-commercial pet movement is clear that this documentary and identity check happens at the point of entry, not at every stop along an onward domestic itinerary (EU rules on travelling with pets). In practice, that means the Rome-to-Catania leg does not require a second veterinary inspection or a new round of customs paperwork. It is domestic transport, similar in principle to a US-based move where a pet clears customs in one city and then continues by car or connecting flight to another.
That said, "no second import check" does not mean "no planning needed." The pet still has to physically get from Rome to Sicily, and that leg has its own logistics, timing, and welfare considerations.
Families and relocation teams generally choose between two options for the Rome-to-Catania leg.
Domestic flight on ITA Airways. ITA Airways operates the domestic route between Rome and Catania. For pets traveling in the cabin, ITA Airways allows a total weight, including carrier and food, of up to 12 kg on domestic flights, compared with 8 kg on international routes, with a carrier no larger than 55 x 40 x 23 cm (ITA Airways, Pets in the Cabin). Dogs that are too large for a standard in-cabin carrier may be eligible for ITA Airways' Large Dog On Board service, which allows dogs up to 30 kg to travel in the cabin on select domestic flights identified in advance as "Large Dog" flights, though this is not guaranteed on every Rome-Catania departure and has to be confirmed directly with ITA Airways (ITA Airways, Large Dog On Board). Larger pets that don't qualify for the cabin travel in the hold, in a rigid fiberglass or plastic carrier that meets ITA Airways' door and ventilation specifications (ITA Airways, Pets as Cargo).
Road transport with a ferry crossing. Sicily is an island, so there is no highway bridge from the mainland. Road transport from Rome to Catania follows the A2 motorway to the toe of Italy, where vehicles, including the pet's transport vehicle, cross the Strait of Messina by car ferry before continuing on to Catania. Mapping services put the driving portion of this route at roughly 795 km and about 8.5 to 9 hours under normal traffic conditions. In practice, experienced pet transport teams often build a longer window into the schedule, closer to 12 to 13 hours, to account for ferry loading and wait times, traffic through Calabria, and planned stops so the pet can be walked and given water along the way.
Neither option is universally "better." A direct domestic flight is faster and involves less time in a moving vehicle, but availability, aircraft type, and cabin space for pets vary by flight. Road transport takes longer but gives the pet more predictable rest breaks and avoids the logistics of a second airport transfer.
No. The animal health certificate and supporting paperwork that got the pet through the entry check in Rome remain valid for the rest of the trip within Italy. There is no second certificate, no second microchip scan by a border authority, and no additional customs declaration required specifically because the final destination is Sicily rather than Rome itself. The documents you prepared for the US-to-Italy leg, following the standard requirements laid out on the USDA APHIS pet travel page for Italy, cover the entire move once the pet has cleared entry (USDA APHIS, Pet Travel: US to Italy). What does change is purely logistical: a new flight or transport booking, and confirmation of any airline-specific requirements for the domestic leg, such as ITA Airways' cabin weight limits or hold carrier specifications.
A transatlantic flight, an entry inspection, and then an immediate second flight or a long drive is a lot to ask of an animal in a single day. Building in a deliberate pause in Rome, even a few hours at a boarding facility for a walk, water, and a chance to decompress, makes a meaningful difference in how a pet handles the rest of the trip. This is especially true for pets connecting onward by road, where a stressed or dehydrated animal makes for a harder 8 to 13 hours in transit.
As Adam Melton, VP of Strategic Initiatives at Starwood Pet Travel, puts it: "The mistake we see most often is people booking their US-to-Rome flight and stopping their planning there, as if the move is finished the moment the pet clears the entry check. Rome is the entry point, not the destination. If your final stop is Sicily, that Rome-to-Catania leg needs its own booking, its own timeline, and its own rest stop for the animal, or the whole trip gets harder than it needs to be."
Stefano Saija, CEO of ReloCat Italy and Starwood's Italy-based partner for the Rome-to-Sicily leg, described how his team approaches it:
"For Sicily, we always look at the calendar and the aircraft first. ITA Airways runs the domestic route to Catania, which is now part of the Lufthansa Group, but not every flight can take a large dog in the cabin. If the flight doesn't work for the pet, we drive, with the ferry crossing at the Strait of Messina built into the schedule from the start, so nobody is guessing on travel day."
Because the Rome entry check and the Rome-to-Catania leg are two separate steps, the most common planning mistake is treating them as one. Build your itinerary with a clear break in Rome, confirm your domestic flight or road transport well ahead of the trip, and check ITA Airways' current cabin and hold requirements directly before booking, since aircraft type and route availability can change. For the full walkthrough of what happens before your pet ever leaves the US, see our complete guide to moving a dog or cat from the US to Italy. You can also review country-specific requirements on our Italy country guide, or request a quote if you'd like help coordinating both legs of the trip.